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JoVE Journal
Bioengineering

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Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement
 

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Article DOI: 10.3791/3164
September 6th, 2011

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Summary September 6th, 2011

Self-assembled monolayers (SAMs) formed from long chain alkane thiols on gold provide well-defined substrates for the formation of protein patterns and cell confinement. Microcontact printing of hexadecanethiol using a polydimethylsiloxane (PDMS) stamp followed by backfilling with a glycol-terminated alkane thiol monomer produces a pattern where protein and cells adsorb only to the stamped hexadecanethiol region.

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