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Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Article DOI: 10.3791/3164 • 08:36 min • September 6th, 2011
September 6th, 2011 •
1, 1, 1
1Department of Chemistry, Washington University in St. Louis

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Self-assembled monolayers (SAMs) formed from long chain alkane thiols on gold provide well-defined substrates for the formation of protein patterns and cell confinement. Microcontact printing of hexadecanethiol using a polydimethylsiloxane (PDMS) stamp followed by backfilling with a glycol-terminated alkane thiol monomer produces a pattern where protein and cells adsorb only to the stamped hexadecanethiol region.

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Microcontact Printing Protein Confinement Cell Confinement Well-defined Substrates Self-assembled Monolayers Protein Patterns Cellular Studies PDMS Stamp Gold-coated Substrate Adhesive Regions Non-adhesive Regions Hydrophobic Surface
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