JoVE Encyclopedia of Experiments
Neuroscience
0 views • 5:09 min • June 17th, 2025
Begin with a diffuse correlation spectroscopy system prepared for measuring cerebral blood flow.
Switch off the laser and detectors.
The system includes a probe composed of source and detector fibers.
Sanitize the probe. Apply double-sided tape, avoiding the optical fibers.
Sanitize the participant’s forehead and place laser safety goggles on the participant.
Secure the probe over the region-of-interest on the participant’s forehead.
Turn on the laser and detectors, then begin cerebral blood flow measurement.
Long-coherent, near-infrared light from the laser penetrates deep into the brain, illuminating the cerebral tissue via the source fiber.
Scattered light from the tissue and moving blood cells in the cerebral microvasculature interfere constructively and destructively.
This interference pattern is recorded by the detector.
Light scattered by moving blood cells causes fluctuations in the interference pattern, which are analyzed to calculate the cerebral blood flow index—a measure of blood flow in the probed cerebral tissue.
Before moving the system into the ICU, connect all the fibers to the relevant lasers and detectors and make sure they are properly attached to the optical probe. Then
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