JoVE Encyclopedia of Experiments
Neuroscience
0 views • 4:05 min • August 8th, 2025
Begin with anesthetized mice placed on a positioning device with their backs parallel to the chair's back support and perpendicular to the seat.
The mice are positioned in a head-down-and-forward posture and secured using chair belts.
Using a micropipette loaded with a neuron-targeting peptide-siRNA complex, place a drop near one nostril, allowing the mouse to inhale the solution naturally.
Gently press the mouse's head downward during inhalation to prevent the solution from draining into the lungs.
Pause briefly, then repeat the administration in the other nostril.
Repeat the procedure for the remaining mice.
The complex penetrates the nasal mucosa, from where it enters the olfactory and trigeminal nerves and travels through them to reach the brain.
Inside the brain, the complex binds to neuronal receptors, undergoes endocytosis, and facilitates intracellular siRNA delivery.
The siRNA becomes part of the RISC complex, which, upon binding to the target mRNA, induces gene silencing.
To begin this procedure, assess the anesthesia level of the mice bipedal reflex in order to maintain the surgical plane. Next, place the positioning device at an appropriate distance and height to allow convenie
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