Intranasal Drug Administration in Mice via Pipette and Reverse-Cannulation for Nose-to-Brain Drug Delivery

Take an anesthetized mouse in a supine position with an inhalation mask to provide anesthesia.

For pipette-based intranasal drug administration, take a micropipette containing a radiolabeled drug.

Insert the micropipette through an opening in the mask and administer the drug into the nasal cavity.

The cavity is lined with respiratory and olfactory epithelium, both of which contain cilia that actively clear substances, thereby limiting drug retention.

The retained drug molecules travel via nerves to reach the brain.

For intranasal drug administration via reverse cannulation, position the mouse under a microscope and make a neck incision.

Expose the trachea, incise it, insert a cannula, and connect it to the mask to maintain anesthesia.

Expose the esophagus, make an incision, and insert a cannula directed towards the nasal cavity.

Ligate the cannula and attach it to a syringe containing the radiolabeled drug.

Deliver the drug against the direction of ciliary motion, enhancing retention and improving drug transport.

For intranasal delivery via micropipette in an isolated radioisotope facility, tape an anesthetized mouse to a cork board in the supine position and administer 25 microliters of ca