JoVE Encyclopedia of Experiments
Neuroscience
0 views • 3:58 min • August 7th, 2025
Secure an anesthetized mouse bearing an implanted tumor onto a stereotactic frame.
Remove the staples from the previous surgery, disinfect the skin, and incise along the previous wound to expose the skull.
Locate the burr hole from the previous surgery to access the brain.
Position the minimally invasive resection system, or MIRS, over the burr hole.
The system consists of a cannula with a rotating window that uses suction to draw the targeted tissue and a cylindrical blade at the aperture to excise the tissue.
Insert the cannula into the target region and initiate resection.
The suction pulls small tumor sections through the window, while the blade excises the tissue.
The rotating window allows controlled resection via continuous suction and cutting.
The excised fragments travel through a sterile tubing to the sample collection chamber.
Withdraw the cannula, close the wound, and allow the mouse to recover.
Begin the experiment by assessing the mouse for sedation by pinching the toe. Apply ophthalmic ointment to the eyes to avoid dryness of the cornea. Then, place the mouse on a stereotactic frame. Remove the staple from the previous surgery and disinfect the skin with alternating cycles of
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