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Chapters
- 00:05Title
- 01:22Media Preparation and Plate Coating
- 02:30Passing and Seeding Undifferentiated Human ES Cells
- 04:27Cardiac Induction in Adhesive Culture with Nicotinamide
- 05:37Differentiation in Suspension Culture and Maturation to Beating Cardiomyocyte Phenotype after Attachment
- 08:05Direct Cardiomyocyte Differentiation of Feeder- and Serum-Free Pluripotent Humand ES Cells with Nicotinamide Induction
- 10:23Conclusion
Vi har etablerat ett protokoll för induktion av cardioblasts direkt från pluripotenta mänskliga embryonala stamceller som upprätthålls enligt fastställda villkor med små molekyler, vilket möjliggör härledning av ett stort utbud av humana stamceller och funktionella cardiomyocytes för kardiovaskulära reparation.
Tags
Human Cardiac PrecursorsCardiomyocytesPluripotent Human Embryonic Stem CellsSmall Molecule InductionRegenerating Human MyocardiumCell-based TransplantationStem/progenitor CellsBone MarrowCord BloodContractile Heart Muscle CellsAdult Stem Cell TherapyGenetically Stable Human Embryonic Stem CellsUnlimited Expansion AbilityUnrestricted PlasticityLarge Supplies Of Human Somatic CellsCardiovascular DiseaseDonor OrgansHESC-based Therapies
