Journal
/
Immunology and Infection
/
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
JoVE Journal
Immunology and Infection
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

DOI:
10.3791/3344-v

•

11:09 min

•

October 23, 2011

•

, , , ,
1Saskatoon Research Centre,Agriculture and Agri-Food Canada, 2Department of Veterinary Microbiology,University of Saskatchewan , 3Plant Biotechnology Institute,National Research Council of Canada

Chapters

  • 00:05Title
  • 03:05Coupling Oligonucleotide Probes to Fluorescent Beads
  • 06:37Production of cpn60 Universal Target Amplicon and Generation of Single Strands
  • 09:36Representative Results

Summary

Automatic Translation

Automatic Translation

We describe a multiplex method for the detection of microorganisms within a sample using oligonucleotide-coupled fluorescent beads. Amplicon from all organisms within a sample is hybridized to a panel of probe-coupled beads. A Luminex or Bio-Plex instrument is used to query each bead for bead type and hybridization signal.

Tags

Multiplex DetectionBacteriaClinical SamplesEnvironmental SamplesOligonucleotide-coupled Fluorescent MicrospheresBacterial VaginosisPolymicrobial SyndromeLactobacillusGardnerella VaginalisAtopobium VaginaeNegative Health OutcomesHIV AcquisitionClinical ManagementDiagnosisGram StainsVaginal Swab SmearsSubjective InterpretationsVaginal Microbiota CompositionDeep SequencingMolecular Diagnosis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/3344?article_type=v

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
check_url/3344?article_type=v

Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples
check_url/3344?article_type=v

Determining the Phagocytic Activity of Clinical Antibody Samples
check_url/3344?article_type=v

Detection of Bacteria Using Fluorogenic DNAzymes
check_url/3344?article_type=v

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
check_url/3344?article_type=v

In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers
check_url/3344?article_type=v

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
check_url/3344?article_type=v

A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis
check_url/3344?article_type=v

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens
check_url/3344?article_type=v

Magnetic Stirrer Method for the Detection of Trichinella Larvae in Muscle Samples

Read Article