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Journal
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Immunology and Infection
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在细胞命运<em> E.大肠杆菌</em感染后的噬菌体λ
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Following Cell-fate in
E. coli
After Infection by Phage Lambda
Please note that all translations are automatically generated.
Click here for the English version.
在细胞命运<em> E.大肠杆菌</em感染后的噬菌体λ
DOI:
10.3791/3363-v
•
06:10 min
•
October 14, 2011
•
Lanying Zeng
,
Ido Golding
2,3
1
Department of Physics
,
University of Illinois at Urbana-Champaign
,
2
Center for the Physics of Living Cells
,
University of Illinois at Urbana-Champaign
,
3
Verna and Marrs McLean Department of Biochemistry and Molecular Biology
,
Baylor College of Medicine
Chapters
00:05
Title
00:58
Creation of a Crude Phage Lysate
01:56
Preparation of Agarose Gel Slabs
02:33
Phage Infection
03:27
Following Cell Fate under the Microscope
04:42
Cycle of Phage Infection
05:45
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了制备荧光标记的版本,λ噬菌体感染的程序<em> E.大肠杆菌</em>细菌,感染后在显微镜下的结果,和感染结果的分析。
Tags
Cell-fate
E. Coli
Infection
Phage Lambda
Lytic Pathway
Lysogenic Pathway
Fluorescent Labeling
Real-time Microscopy
Individual Phages
Individual Cells
Infection Experiments
Fluorescent Phages
Capsid GpD Protein
YFP-fusion
Crude Phage Lysate
Purification Steps
DAPI-labeling
Microscope Imaging
Adsorption
Viral DNA Injection
Article
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