Journal
/
/
Roterende Cell Culture Systems for Human Cell Culture: Human trofoblasten celler som en model
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model
DOI:

06:54 min

January 18, 2012

, , , ,

Chapters

  • 00:05Title
  • 01:04EVT Cells and Collagen Bead Incubation
  • 02:13Loading the Rotating Cell Culture System (RCCS)
  • 03:41Changing the Media
  • 04:37Collecting Propagated Cells
  • 05:39SGHPL-4 Trophoblast Cells in a 3D Milieu
  • 06:30Conclusion

Summary

Automatic Translation

Traditionel, todimensionale cellekultur teknikker ofte resultere i ændrede egenskaber med hensyn til differentiering markører, cytokiner og vækstfaktorer. Tre-dimensionel cellekultur i den roterende cellekultur-system (RCCS) genopretter udtryk for mange af disse faktorer, som vist her med en extravillous trofoblasten cellelinje.

Related Videos

Read Article