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Bioengineering

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JoVE Journal Bioengineering
Roterande Cell Culture System för mänskliga Cell Culture
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Journal (Bioengineering)
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Journal (Bioengineering)
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Roterande Cell Culture System för mänskliga Cell Culture: Mänskliga trofoblasten Celler som modell

Article DOI: 10.3791/3367-v • 06:54 min • January 18th, 2012
January 18th, 2012 •
*1, *1,2, 3, 1, 1
1Department of Microbiology and Immunology, Tulane University Medical School, 2Physician/Scientist Program, Tulane University Medical School, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
* These authors contributed equally

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Traditionell, tvådimensionell cellodling tekniker leder ofta till förändrade egenskaper vad gäller differentiering markörer, cytokiner och tillväxtfaktorer. Tredimensionella cellodling i roterande cellodlingssystem (RCC) återupprättar uttrycket av många av dessa faktorer så som visas här med en extravillous trofoblasten cellinje.

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Bioteknik Extravillous trophoblasts cytotrophoblast invasion matrix metalloproteinas 3-D cellodling RCC ECM microcarriers
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