Morphometric Analyses of Retinal Sections

Hi, I am Regina from the laboratory of the Neuro Degenerative Disease in the Department of Anatomy, the University of Hong Kong. Today I'm gonna show you how to do the progression analysis of the Retinal session. Let's get started Before doing any analysis, a retinal sample recession and four MyFitness and undergo the h and e staining.

After that, the retinal session is divided into four regions. They are the upper peripheral region, the upper central region, the lower central region, and the lower peripheral region. After defining the regions, an image of each region is acquired at 10 times 40 magnification under the bright beam microscope of decor.

Now we are ready to analyze the retinal morph informatory. This session will demonstrate how to measure the retinal thick of the inner nuclear layer. Open the software called thorough investigator.

Choose the 40 types, then from the main two bar select image open. Under the tab file, choose an image of a desired retinal region that you would like to analyze. Choose the type of contour line from the dropdown of the main two bar.

Ensure the auto move is turned on. You can enlarge the image by clicking zoom in button. You can use zoom out button to reduce the side of the image display.

Both the tracing and the specimen can be moved along four different direction by cracking the cause movement buttons. Right click in the tracing window and select simple crack tracing. Draw the cont line along the inner border of the inner layer.

If a mistake is made, break it in the tracing window and press the und undo button to erase the last drawing point. Continue the tracing until the last point of the line. When the drawing is done, break it in the tracing window and choose an open contour and launch the image by clicking zoom in.

Button left Click in the tracing window. To enlarge the image, select a marker at the marker two bar at Marcus. At the junction point.

After adding all the markers, the select the marker icon at the marker two bar position and cursor. At the center of one of the markers, select quick measure angles under the tab twos right click in the tracing window and the select the continuous option. Measure angle by first clicking at the center of a marker, which is adjacent to the marker selected.

For an end of the line segment, move the most to the center of the concern. Marker and crack. Again, extend the rubber band line to the outer border of the inia layer at adjust the angle until 90 degrees.

Reach left crack at the desire. Position a dialog both showing the angle measurement will pop up right after the click. Press the enter key while holding the mouse still left.

Click the tracing window so the line is drawn from one end to the new select end. Break it in the tracing window and choose an open control. We have to take four measurements of the inner nuclear layer fingers in each region, where four lines will be drawn.

Perpendicularly from the control base night to the outer border of the India nuclear layer And launch the image so that you can see the border clearly. If the tip of the line is not at the outer of the inner nuclear layer, we can either link them or shorten the line under the editing mode. Select the editing mode and pick the line.

Very click the tracing window and choose in search point in electric control, add a point at the outer border while the point edit should be along the same line. Delete the original point. Align with a new lift can then be obtained.

Reduce the side of the image by clicking the zoom out button. De select the editing mode and draw the last line segment. After drawing order lines, select the editing mode.

Right click the tracing window and we land the four line segments accordingly. We can now obtain the four measurements by pressing the CONT measurement button. Select these four measurements and paste them to a specialist by clicking copy.

To click ball select save as under the tab file. The tracing data can be saved as a data file. In this part, we will quantify the number of red tail gagan cell select image open.

Under the tab file, choose an image of a desired retinal region that you would like to analyze. First of all, we have to identify which are lifestyle and which are death cells. Those cells in a roughly run shape with subtle and nucleus clearly observed unconsidered as life's retinal gang cells.

Those compare be small and condensed cells are considered as that cell. Choose the type of contour line from the dropdown of the main two bar. Draw aour line along the border of left fiber layer and the tracing with the option and open contour.

Add each end, add one marker at the tip of the line and the other at the position near the team. You select the marker icon at the marker two bar. Position the cursor at the center of the markers.

At the tip, select quick measure angles under the tab. Twos right click in the tracing window and you select a continuous option and just the rubber band lines and measure an angle of 19 degree. Press the enter key right after a dollar box is caught up.

But remember the mouse is have still at the same time left. Break the tracing window so that a new line can be particularly extended from the tip. Break in the tracing window and choose an open control.

Draw another new line in the exactly the same way as another end. We have created two boundaries within which the number of ratin gagan cells is counted. Select one type of marker and attach one to each five cell.

Choose one boundary as an exclusion line, which the cell touches is not current. Select another type of marker for the dead cells. The number of each type of marker space will be dis displayed alongside the marker icon.Accordingly.

At the marker two bar, select the editing mode and pick up the contour baseline. Break it in the tracing window and re end the line. The name of the contour baseline can be obtained by pressing contour measurement button.

The number of the cells can then be expressed per millimeter of the name. So let's save as under the tab file. The tracing and the marker data can be saved as an data file.

This part will demonstrate how to measure the cell side of a retinal ganging cell select image open. Under the tab file, choose an image of a desired retinal region that you would like to analyze. Choose a live breath gang cell according to the criteria described in the previous part.

So in the life cell, choose the type of contour line from the jot down spot of the main two bar. Draw a contour line along the circumference of the cell at about the end right kick the tracing window and select end close contour. The area of the cell will be displayed under the item of area in the control measurement dialogue box.

This video has demonstrated three simple methods that can objectively analyze the retinal. Hopefully this video is of.