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Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas

Article DOI: 10.3791/3403-v • 09:47 min • November 6th, 2011
November 6th, 2011 •
1, 2, 2, 1,2
1Neuro-Oncology Research, Barrow Neurological Institute of St. Joseph’s Hospital and Medical Center, 2Neurosurgery Research Laboratory, Barrow Neurological Institute of St. Joseph’s Hospital and Medical Center

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Summary
Automatic Translation

Intracranial implantation of GL261 cells into C57BL/6 mice produces malignant gliomas that recapitulate many of the hallmarks of human glioblastoma multiforme. We used GL261 cells stably expressing luciferase to allow us to use in vivo imaging to follow tumor progression. The surgery and 3D in vivo imaging are demonstrated.

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Intracranial Implantation 3D In Vivo Bioluminescent Imaging Murine Gliomas GL261 Glioblastoma Multiforme Intracranial Mouse Tumor Model Firefly Luciferase GL261-luc GL261-luc2 C57BL/6-cBrd/cBrd/Cr Mice Albino Variant In Vivo Imaging IVIS Spectrum Caliper Life Sciences Histological Features Tumor Necrosis Pseudopalisades Neovascularization Invasion Hypercellularity Inflammation
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