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Detección de interacciones de proteínas en la planta mediante un Gateway Compatible Complementación fluorescencia bimoleculares (BiFC) del sistema
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Summary September 16th, 2011
Please note that all translations are automatically generated.
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Hemos desarrollado una técnica para probar interacciones proteína-proteína en las plantas. Una proteína amarilla fluorescente (YFP) se divide en dos fragmentos que no se superponen. Cada fragmento se clona en el marco de un gen de interés a través del sistema de puerta de enlace, lo que permite la expresión de proteínas de fusión. La reconstitución de la señal de YFP sólo ocurre cuando las proteínas interactúan investigación.
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