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JoVE Journal
Biology
Author Produced

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Identification of Protein Interacting Partners Using Tandem Affinity Purification
 

Identification of Protein Interacting Partners Using Tandem Affinity Purification

Article DOI: 10.3791/3643
February 25th, 2012

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Summary February 25th, 2012

Tandem affinity purification is a robust approach for the identification of protein binding partners. As proof of concept, this methodology was applied to the well-characterized translation initiation factor eIF4E to co-precipitate the host cell factors involved in translation initiation. This method is easily adapted to any cellular or viral protein.

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