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Enkel og robust In vivo Og In vitro Approach for å studere Virus Assembly
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Chapters
- 00:05Title
- 03:16Delivery and Expression of Functional Viral Genome Components to Plant Cells by Agroinfiltration
- 06:00Preparation of Capsid Protein Subunits for in vitro Assembly
- 07:07In vitro Assembly of RNA-containing Virions
- 08:48Representative Viral Assembly Results
- 09:22Conclusion
En enkel, effektiv og robust måte å synkronisere levering av flere virale komponenter til planteceller via<em> Agrobacterium</em>-Mediert forbigående uttrykk er beskrevet. Denne tilnærmingen er mottagelig for å studere replikering, etterfulgt encapsidation av<em> In vitro</em> Remontering av ikke-virale komponenter i genomet utarmet optiske virale spøkelser egnet for biomedisinske applikasjoner.
Tags
Virus AssemblyIn VivoIn VitroEncapsidationPositive-sense RNA GenomesInfectious VirionsMechanism Regulating Virus AssemblyCurbing Virus SpreadDisease ControlMacromolecular InteractionsSubcellular CompartmentalizationBiomedical ImagingTherapeutic ApplicationsNon-enveloped Plant VirusesBrome Mosaic Virus (BMV)Genome PackagingNicotiana Benthamiana PlantsAgroinfiltration
