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Immunohistological Labeling of Microtubules in Sensory Neuron Dendrites, Tracheae, and Muscles in the Drosophila Larva Body Wall
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Immunohistological Labeling of Microtubules in Sensory Neuron Dendrites, Tracheae, and Muscles in the Drosophila Larva Body Wall
DOI:

07:49 min

November 10, 2011

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Chapters

  • 00:05Title
  • 01:11Larval Dissection
  • 02:56Fixation of Larval Fillets
  • 03:59Blocking, Staining, and Mounting of Larval Fillets
  • 05:56Fluorescent Staining
  • 07:22Conclusion

Summary

Automatic Translation

To understand how complex cell shapes, such as neuronal dendrites, are achieved during development, it is important to be able to accurately assay microtubule organization. Here we describe a robust immunohistological labeling method to examine microtubule organization of dendritic arborization neuron sensory dendrites, trachea, muscle, and other Drosophila larva body wall tissues.

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