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JoVE Journal
Immunology and Infection

This content is Open Access.

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes
 

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

Article DOI: 10.3791/3709
June 28th, 2012

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Summary June 28th, 2012

Temporal and spatial gene expression analyses have a crucial role in functional genomics. Whole-mount hybridization in situ is useful for determining the localization of transcripts within tissues and subcellular compartments. Here we outline a hybridization in situ protocol with modifications for specific target tissues in mosquitoes.

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