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Journal / Immunology and Infection / Um sistema de cultura primária Neuron para o…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Um sistema de cultura primária Neuron para o Estudo da Herpes Simplex Virus latência e reativação
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Journal (Immunology and Infection)
A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay
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Um sistema de cultura primária Neuron para o Estudo da Herpes Simplex Virus latência e reativação

Article DOI: 10.3791/3823-v • 12:22 min • April 2nd, 2012
April 2nd, 2012 •
1, 1, 2, 3, 2,4,5,6,7, 1, 1
1Department of Microbiology, New York University School of Medicine, 2Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine, New York University School of Medicine, 3Department of Otolaryngology, New York University School of Medicine, 4Department of Cell Biology, New York University School of Medicine, 5Department of Physiology and Neuroscience, New York University School of Medicine, 6Department of Psychiatry, New York University School of Medicine, 7Center for Neural Science, New York University School of Medicine

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O protocolo descreve um sistema de modelo eficiente e reprodutível para estudar vírus herpes simplex tipo 1 (HSV-1) a latência e reactivação. O ensaio emprega homogéneos culturas de neurónios simpáticos e permite a dissecção molecular do vírus de neurónios-interacções usando uma variedade de ferramentas, incluindo a interferência de RNA e de expressão de proteínas recombinantes.

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Immunology Edição 62 neurónio cultura de células Vírus Herpes Simplex (HSV) biologia molecular virologia
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