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Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae
This content is Free Access.
1Department of Biochemistry and Molecular Biology,The University of Texas MD Anderson Cancer Center, 2Scholars Academy/MARC Scholar,University of Houston-Downtown, 3Genes and Development Graduate Program,University of Texas Graduate School of Biomedical Sciences, 4Neuroscience Graduate Program,University of Texas Graduate School of Biomedical Sciences
Chapters
- 00:05Title
- 02:02Preparation of Larvae
- 02:37Etherization and UV Irradiation
- 04:11Local Heat Probe Assay
- 05:23Global Heat Plate Assay
- 07:49Results: Quantified Behavioral Responses in Local and Global Thermal Nociception Assays
- 10:15Conclusion
In this article, we demonstrate assays to study thermal nociception in Drosophila larvae. One assay involves spatially-restricted (local) stimulation of thermal nociceptors1,2 while the second involves a wholesale (global) activation of most or all such neurons3. Together, these techniques allow visualization and quantification of the behavioral functions of Drosophila nociceptive sensory neurons.
