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Candida albicans Biofilm Chip (CaBChip) for High-throughput Antifungal Drug Screening
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Chapters
- 00:05Title
- 01:08Preparation of Functionalized Slides
- 02:30Preparation of Yeast Inocula and Collagen Encapsulation
- 03:40Preparation of CaBChip
- 04:57Susceptibility Testing of Preformed Biofilms in CaBChip against Antifungal Agents
- 06:38Representative Biofilm Chip Results
- 07:48Conclusion
We have developed a high-density microarray platform consisting of 3D nano-biofilms of C. albicans called CaBChip. The susceptibility profile of drugs tested on a CaBChip is comparable to the conventional 96-well plate model, suggesting that the fungal chip is ideally suited for true high-throughput screening of antifungal drugs.
Tags
Candida AlbicansBiofilm ChipCaBChipHigh-throughputAntifungal Drug ScreeningCandidiasisNosocomial Bloodstream InfectionCompromised IndividualsMortality RatesAntifungal DrugsResistanceBiofilmsMicroarrayC. Albicans Nano-biofilmsRobotic MicroarrayerYeast CellsSolid SubstrateThree-dimensional MatrixGlass SubstrateCoatingIncubationPhenotypic Characteristics
