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Harvesting Murine Alveolar Macrophages and Evaluating Cellular Activation Induced by Polyanhydride Nanoparticles
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Bioengineering
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JoVE Journal Bioengineering
Harvesting Murine Alveolar Macrophages and Evaluating Cellular Activation Induced by Polyanhydride Nanoparticles

Harvesting Murine Alveolar Macrophages and Evaluating Cellular Activation Induced by Polyanhydride Nanoparticles

DOI:
10.3791/3883-v

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11:26 min

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June 08, 2012

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, , , ,
1Department of Chemical and Biological Engineering,Iowa State University, 2Department of Veterinary Microbiology and Preventive Medicine,Iowa State University

Chapters

  • 00:05Title
  • 01:40Harvesting Alveolar Macrophages from the Mouse using Lung Lavage
  • 04:26Processing of Lung Lavage Harvest
  • 06:29Addition of Polyanhydride Nanoparticles and Control Treatments
  • 08:59Results from Flow-cytometric Analysis of Nanoparticle-induced Activation of Isolated Alveolar Macrophages
  • 10:46Conclusion

Summary

Automatic Translation

Automatic Translation

Herein, we describe protocols for harvesting murine alveolar macrophages, which are resident innate immune cells in the lung, and examining their activation in response to co-culture with polyanhydride nanoparticles.

Tags

Murine Alveolar MacrophagesCellular ActivationPolyanhydride NanoparticlesIntranasal VaccinesRespiratory Infectious DiseasesSebacic Acid16-bis(p-carboxyphenoxy)hexane18-bis(p-carboxyphenoxy)-36-dioxaoctaneBulk Erosion KineticsSurface Erosion KineticsBiomolecules ReleaseAdjuvant ActivityVaccine Delivery PlatformInnate ImmunityMolecular ResponsesCellular ResponsesAntigen Presenting Cells (APCs)Dendritic CellsAlveolar Macrophages (AM)Lung ParenchymaMicrobial PathogensCell Debris

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