Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

JoVE Journal Biology
Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water
Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells
Journal (Biology)
Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells
Organotypic Slice Culture of E18 Rat Brains
Journal (Biology)
Organotypic Slice Culture of E18 Rat Brains
 

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

Article DOI: 10.3791/3891
February 20th, 2013 •
1, 1
1Boston Biomedical Research Institute

Chapters

Summary
Automatic Translation
February 20th, 2013

Stable isotope labeling workflows employing 18O-enriched water (LeO-workflows) are versatile tools for quantitative and qualitative proteomics studies. In protease-assisted (PALeO) workflows, 18O-atoms are introduced by proteolytic cleavage and carboxyl oxygen exchange reactions mediated by proteases. In the acid-catalyzed (ALeO) workflow, 18O-atoms are introduced by carboxyl oxygen exchange at low pH.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter