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Chapters
- 00:05Title
- 02:04Anesthesia and Monitoring
- 08:00Preparation of the Cremaster Muscle
- 11:27Intravital Setup
- 13:15Offline Video Playback Analysis
- 13:59Conclusion
Digital intravital epifluorescence microscopy of postcapillary venules in the cremasteric microcirculation is a convenient method to gain insights into leukocyte-endothelial interaction in vivo in ischemia-reperfusion injury (IRI) of striated muscle tissue. We here provide a detailed protocol to safely perform the technique and discuss its applications and limitations.
Tags
Real-timeDigital ImagingLeukocyte-endothelial InteractionIschemia-reperfusion InjuryIRIRat Cremaster MusclePathological ConditionsCerebral StrokeMyocardial InfarctionIntestinal IschemiaTransplantCardiovascular SurgeryReactive Oxygen SpeciesComplement SystemMicrovascular PermeabilityLeukocyte ActivationAdhesion MoleculesChemoattractantsSelectinsCounter LigandsIntercellular Adhesion MoleculesVascular Cellular Adhesion MoleculesIntravital Microscopy
