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Chapters
- 00:05Title
- 01:24Preparing Transgenic Mice
- 02:08Intraductal Injection of LPS
- 05:20Bioluminescent Imaging to Visualize NF-kB Activity
- 06:29Image Analysis
- 07:27Practice Injection Results and IVIS Images
- 08:29Conclusion
Beskrevet her er en teknik, hvor lipopolysaccharid injiceres i lakterende muse brystkirtel via brystvorten at simulere mastitis, en betingelse, der almindeligvis forårsaget af bakteriel infektion. Lipopolysaccharid injektion resulterer i øget nuclear factor kappa B (NF-KB) signalering, visualiseret ved bioluminescerende billeddannelse af en NF-KB luciferase reporter mus.
Tags
Intraductal InjectionLPSMouse ModelMastitisNF-κB ReporterTransgenic AnimalDisease ProgressionPre-clinical ModelsLipopolysaccharideLactating Mouse Mammary GlandSimulated InfectionInflammationNuclear Factor Kappa BBioluminescent ImagingLuciferase Reporter MouseRednessSwellingImmune Cell InfiltrationStraight-forward MethodInsulin SyringeMammary Duct
