Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
 

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Article DOI: 10.3791/4045
May 22nd, 2012

Chapters

Summary May 22nd, 2012

Tracking subtle changes in the progression and kinetics of cell cycle stages can be accomplished by use of a combination of metabolic labeling of nucleic acids with BrdU and total genomic DNA staining via Propidium Iodide. This method avoids the need of chemical synchronization of cycling cells, thereby preventing the introduction of non-specific DNA damage, which in turn affects cell cycle progression.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter