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冷冻干燥细菌及其影响细胞生存的配方
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Biology
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JoVE Journal
Biology
Formulations for Freeze-drying of Bacteria and Their Influence on Cell Survival
Please note that all translations are automatically generated.
Click here for the English version.
冷冻干燥细菌及其影响细胞生存的配方
DOI:
10.3791/4058-v
•
08:55 min
•
August 03, 2013
•
Per Wessman
,
Sebastian Håkansson
,
Klaus Leifer
,
Stefano Rubino
1
Department of Microbiology, Uppsala Biocenter
,
Swedish University of Agricultural Sciences
,
2
Department of Engineering Sciences
,
Uppsala University
Chapters
00:05
Title
00:46
Cultivation and Harvest of P. putida
01:37
Formulation Solutions, Preparing Cells for Freeze-drying, and Surface Tension Measurements
03:22
Characterization of Formulation Freezing Behavior by Differential Scanning Calorimetry (DSC) and Freeze-drying
05:31
X-ray Analysis of the Dry Formulations and Electron Microscopy
06:38
Representative Freeze-drying Results
08:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
冷冻干燥往往是一个简单方便的方式来获得可行的细菌细胞的干制品。一个问题的过程,是细胞的存活。我们这里详细调查冷冻干燥过程中的细胞的存活的影响所用的公式的属性的一个过程。
Tags
Formulations
Freeze-drying
Bacteria
Cell Survival
Dehydration Method
Matrix
Protection
Sucrose
Ficoll PM400
Hydroxyethyl Cellulose
Hydroxypropyl Methyl Cellulose
P. Putida KT2440
A. Chlorophenolicus A6
Rehydration
Characterizing
Differential Scanning Calorimetry (DSC)
Surface Tension Measurements
X-ray Analysis
Electron Microscopy
Survival Rates
Polymers
Article
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