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O Processo de monstro verde para a Geração de linhagens de levedura de transporte deleções de genes múltiplos

Article DOI: 10.3791/4072 • 13:06 min
December 15th, 2012 •
1, 1, 2, 3, 2, 3,4
1Department of Synthetic Biology and Bioenergy, J. Craig Venter Institute, 2Department of Microbial and Environmental Genomics, J. Craig Venter Institute, 3Donnelly Centre & Department of Molecular Genetics, University of Toronto, 4Lunenfeld Research Institute, Mt Sinai Hospital

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December 15th, 2012

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O método permite que o monstro verde montagem rápida de exclusões múltiplas marcados com um gene que codifica a proteína verde fluorescente repórter. Este método baseia-se em estirpes de levedura de condução através de ciclos repetidos de sortimento sexual de deleções e fluorescência baseada enriquecimento de células que transportem mais deleções.

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