12,405 Views
•
09:25 min
•
June 19, 2012
DOI:
Summary
Automatically generated
This article describes a method for the isolation and purification of intact Legionella-containing vacuoles (LCVs) from amoeba and macrophages. The two-step protocol comprises LCV enrichment by immuno-magnetic separation using an antibody against a bacterial LCV marker and further purification by density gradient centrifugation.
Explore More Videos
Related Videos
07:15
Invasion of Human Cells by a Bacterial Pathogen
Related Videos
34675 Views
08:30
One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
Related Videos
25244 Views
05:31
Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Related Videos
34941 Views
11:20
Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
Related Videos
13017 Views
08:52
Live-cell Video Microscopy of Fungal Pathogen Phagocytosis
Related Videos
38898 Views
06:57
Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens
Related Videos
26843 Views
10:42
RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells
Related Videos
9130 Views
07:46
Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
Related Videos
25039 Views
08:34
Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes
Related Videos
8466 Views
14:26
Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)
Related Videos
10038 Views
Read Article
Cite this Article
Hoffmann, C., Finsel, I., Hilbi, H. Purification of Pathogen Vacuoles from Legionella-infected Phagocytes. J. Vis. Exp. (64), e4118, doi:10.3791/4118 (2012).
Copy