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Purification of Pathogen Vacuoles from Legionella-infected Phagocytes
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Chapters
- 00:05Title
- 01:23Preparations for the Production of Legionella pneumophila-containing Vacuoles (LCVs)
- 02:31LCV Isolation
- 06:46Microscopic Analysis of the Collected Samples
- 07:38Representative Results
- 09:00Conclusion
This article describes a method for the isolation and purification of intact Legionella-containing vacuoles (LCVs) from amoeba and macrophages. The two-step protocol comprises LCV enrichment by immuno-magnetic separation using an antibody against a bacterial LCV marker and further purification by density gradient centrifugation.
Tags
Pathogen VacuolesLegionella-infected PhagocytesLegionella PneumophilaLegionnaires’ DiseaseLegionella-containing Vacuole (LCV)Icm/Dot Type IV Secretion SystemEffector ProteinsHost ProteinsLipidsOrganellesLCV FormationIntegrative ApproachProteomicsLipidomicsLiquid Chromatography2-D Gel ElectrophoresisMass-spectrometryDictyostelium DiscoideumLeishmaniaListeriaMycobacteriumRhodococcusSalmonella
