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Immunology and Infection

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JoVE Journal Immunology and Infection
Purification of Pathogen Vacuoles from Legionella-infected Phagocytes
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Purification of Pathogen Vacuoles from Legionella-infected Phagocytes

Article DOI: 10.3791/4118
June 19th, 2012 •
1, 1, 1
1Max von Pettenkofer-Institut, Ludwig-Maximilians-Universität

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June 19th, 2012

This article describes a method for the isolation and purification of intact Legionella-containing vacuoles (LCVs) from amoeba and macrophages. The two-step protocol comprises LCV enrichment by immuno-magnetic separation using an antibody against a bacterial LCV marker and further purification by density gradient centrifugation.

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