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Neuroscience

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Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

Article DOI: 10.3791/4141
August 17th, 2012 •
1, 1,2
1Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, 2Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine

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August 17th, 2012

An in vitro model for genetic study of axon regeneration using cultured adult mouse dorsal root ganglion neurons is described. The method includes a re-suspension/re-plating step to allow axon re-growth from neurons undergoing genetic manipulation. This approach is especially useful for loss-of-function studies of axon regeneration using RNAi-based protein knockdown.

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