26,520 Views
•
09:42 min
•
August 17, 2012
DOI:
Summary
Automatically generated
An in vitro model for genetic study of axon regeneration using cultured adult mouse dorsal root ganglion neurons is described. The method includes a re-suspension/re-plating step to allow axon re-growth from neurons undergoing genetic manipulation. This approach is especially useful for loss-of-function studies of axon regeneration using RNAi-based protein knockdown.
Related Videos
09:17
Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration
Related Videos
21223 Views
08:23
In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model
Related Videos
8981 Views
09:48
An Approach to Enhance Alignment and Myelination of Dorsal Root Ganglion Neurons
Related Videos
8413 Views
09:48
Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration
Related Videos
17649 Views
09:00
Laminotomy for Lumbar Dorsal Root Ganglion Access and Injection in Swine
Related Videos
12643 Views
08:15
Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release
Related Videos
48943 Views
06:17
Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion
Related Videos
8131 Views
00:09
Use of In Vivo Single-fiber Recording and Intact Dorsal Root Ganglion with Attached Sciatic Nerve to Examine the Mechanism of Conduction Failure
Related Videos
8693 Views
07:22
Rapid Isolation of Dorsal Root Ganglion Macrophages
Related Videos
10082 Views
06:53
Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments
Related Videos
4943 Views
Read Article
Cite this Article
Saijilafu, Zhou, F. Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons. J. Vis. Exp. (66), e4141, doi:10.3791/4141 (2012).
Copy