Adult Drg Neurons
Adult Drg Neurons - Related Videos
03:44
Co-Culture of Schwann Cells with DRG Neurons on a Pre-Stretched Membrane
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2025
The video demonstrates the co-culturing of dorsal root ganglion (DRG) neurons with Schwann cells. DRG neurons are first grown on a pre-stretched membrane for aligned axon growth. Schwann cells are then introduced, which attach to the axons, insulating them and promoting neural cell survival and function.
09:14
DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord
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Cited by 20 •
2011
The stereotyped projections of sensory afferents into the rodent spinal cord offer an easily accessible experimental system to study axonal branching through the tracing of single axons.
04:25
Isolation of DRG Neurons and Coculture with Schwann Cell Precursors to Generate Schwann Cells
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2025
This video demonstrates a procedure for isolating dorsal root ganglion (DRG) neurons from rat embryos and co-culturing them with Schwann cell precursors to generate mature Schwann cells. The isolated DRGs are processed to isolate neurons and glial cells. The isolated cells are cultured to purify the neurons, which are then co-cultured with Schwann cell-like cells (SCLCs) in a co-culture medium. Interaction with the DRG neurons leads to the maturation of SCLCs into Schwann cells.
09:42
Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons
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Cited by 38 •
2012
An in vitro model for genetic study of axon regeneration using cultured adult mouse dorsal root ganglion neurons is described. The method includes a re-suspension/re-plating step to allow axon re-growth from neurons undergoing genetic manipulation. This approach is especially useful for loss-of-function studies of axon regeneration using RNAi-based protein knockdown.
09:00
Studying the Integration of Adult-born Neurons
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Cited by 11 •
2011
A way to study the integration of newborn dentate granule cells in adult animals is described. This technique uses an engineered retrovirus to label newborn neurons, followed by electrophysiological recordings to determine in vivo functional integration.
FAQs
The pseudounipolar layout lets researchers examine two linked stages of sensory communication within one mature cell: a peripheral axon receives information from temperature, touch, or tissue damage, while a central axon carries encoded action potentials toward the spinal cord. This arrangement helps connect stimulus detection with central transmission when analyzing sensory signaling in neuroscience.
Cultured cells provide an accessible setting for studying ion channel function, axonal transport, and regeneration after injury. These processes represent distinct aspects of neuronal behavior: ion channels relate to signaling, axonal transport concerns movement along the neuron, and regeneration addresses responses to damage. Examining them together supports a broader view of sensory neuron function.
Their role in transmitting information about tissue damage makes adult DRG neurons relevant to studies of pain signaling and chronic pain. Because these cells also participate in peripheral nerve responses and regeneration after injury, they help researchers investigate how sensory pathways are affected by neural damage and how potential neuroprotective treatments or repair strategies might be evaluated.
Cultured adult DRG neurons offer researchers an accessible model for examining mature sensory-cell properties outside the intact nervous system. Investigators can use this model to study sensory signaling, ion channel function, axonal transport, and regeneration after injury. The range of processes available in one system supports experiments connecting cellular mechanisms with broader neuroscience questions.
Studies of these neurons can reveal how sensory information is handled at the cellular level and how neuronal processes change after injury. Results may inform research on chronic pain, peripheral neuropathy, neuroprotective treatments, and nerve repair. The model therefore links observations of neuronal function with conditions and interventions involving sensory nerves.
Adult DRG neurons connect sensory-neuron biology with investigations of peripheral nerve damage. Researchers can examine signaling, axonal transport, and regeneration after injury in cultured cells, then use the findings to support work on peripheral neuropathy, neuroprotection, and nerve repair. This makes the model relevant both to mechanism-focused neuroscience and to therapeutic research goals.