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1Medications Development, Ernest Gallo Clinic and Research Center,University of California, San Francisco , 2Clinical Pharmacology and Experimental Therapeutics,University of California, San Francisco , 3Translational Research Institute and the Institute for Health and Biomedical Innovation,Queensland University of Technology, Brisbane, Australia
Chapters
- 00:05Title
- 01:19Cell Treatment
- 04:36Acquisition of Three-dimensional Fluorescence Images
- 05:26Three-dimensional Morphometric Analysis
- 07:31Results: Single-cell Phenotypic Changes Visualized Through Three-dimensional Morphometric Analysis
- 09:29Conclusion
We developed a software platform that utilizes Imaris Neuroscience, ImarisXT and MATLAB to measure the changes in morphology of an undefined shape taken from three-dimensional confocal fluorescence of single cells. This novel approach can be used to quantify changes in cell shape following receptor activation and therefore represents a possible additional tool for drug discovery.
Tags
Analytical ToolCellular Morphology ChangesThree-dimensional Fluorescence ImagesSoftware Analysis ToolsFluorescence Image Measurement3D DatasetsCellular DynamicsCellular PlasticityMicroscopy InstrumentsMultispectral Fluorescence ImagesConfocal Stacks3D RepresentationDesign-based Stereology MethodsAutomated Analytic MethodCell Morphology AnalysisProtein Localization AnalysisReceptor Trafficking AnalysisMeta-Morph
