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Glycan Profiling of Plant Cell Wall Polymers using Microarrays
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Glycan Profiling of Plant Cell Wall Polymers using Microarrays

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

DOI:
10.3791/4238-v

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12:30 min

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December 17, 2012

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, , , , ,
1Australian Centre of Excellence in Plant Cell Walls, School of Botany,University of Melbourne, 2Plant Cell Biology Research Centre, School of Botany,University of Melbourne, 3CSIRO Plant Industry, Black Mountain Laboratories, 4Department of Plant Biology and Biotechnology,University of Copenhagen

Chapters

  • 00:05Title
  • 01:40Tissue Collection & Preparation
  • 03:01Extraction of Cell Wall Glycans
  • 04:20Printing Microarrays
  • 05:45Probing of Glycan Microarrays
  • 06:36Quantification
  • 07:49Results: CoMPP Profiling of N. alata
  • 11:17Conclusion

Summary

Automatic Translation

Automatic Translation

A technique called Comprehensive Microarray Polymer Profiling (CoMPP) for the characterisation of plant cell wall glycans is described. This method combines the specificity of monoclonal antibodies directed to defined glycan-epitopes with a miniature microarray analytical platform allowing screening of glycan occurrence in a broad range of biological contexts.

Tags

Glycan ProfilingPlant Cell Wall PolymersMicroarraysHeterogeneous GlycansBiosynthesisFunctionGlycosyl ResiduesLinkagesRing StructureIsomeric ConfigurationAnomeric ConfigurationNon-sugar ResiduesDevelopmentEnvironmental CuesGenesArabidopsisReverse Genetics Approaches

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