Journal
/
Neuroscience
/
Manual Drainage of the Zebrafish Embryonic Brain Ventricles
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Manual Drainage of the Zebrafish Embryonic Brain Ventricles

Manual Drainage of the Zebrafish Embryonic Brain Ventricles

DOI:
10.3791/4243-v

•

06:17 min

•

December 16, 2012

•

,
1Department of Biology, Whitehead Institute of Biomedical Research,Massachusetts Institute of Technology

Chapters

  • 00:05Title
  • 01:21Preparing Microinjection Needles, Oil Injector Apparatus, and Embryos
  • 02:28Draining and Collecting the Embryonic Cerebrospinal Fluid (eCSF)
  • 04:24Representative eCSF Drainage Results
  • 05:55Conclusion

Summary

Automatic Translation

Automatic Translation

We present a method to collect cerebrospinal fluid (CSF) and to create a system which lacks CSF within the embryonic zebrafish brain ventricular system. This allows for further examination of CSF composition and its requirement during embryonic brain development.

Tags

Manual DrainageZebrafish Embryonic Brain VentriclesCerebrospinal FluidProtein-rich FluidVertebrate Embryonic DevelopmentChoroid PlexusHighly Vascularized Secretory RegionsNeuroepitheliumEmbryonic CSFECSFAdult CSFSecreted FactorsNeurogenesisGene ExpressionCell ProliferationCell SurvivalProteomic Analyses

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/4243?article_type=v

An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
check_url/4243?article_type=v

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides
check_url/4243?article_type=v

Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells
check_url/4243?article_type=v

A Comprehensive Protocol for Manual Segmentation of the Medial Temporal Lobe Structures
check_url/4243?article_type=v

Isolation and Culture of Endothelial Cells from the Embryonic Forebrain
check_url/4243?article_type=v

Electrophysiological Recording in the Brain of Intact Adult Zebrafish
check_url/4243?article_type=v

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex
check_url/4243?article_type=v

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain
check_url/4243?article_type=v

Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain
check_url/4243?article_type=v

Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal

Read Article