11,726 Views
•
12:34 min
•
December 13, 2012
DOI:
Summary
Automatically generated
A method of observing individual DNA molecules in live cells is described. The technique is based on the binding of a fluorescently tagged lac repressor protein to binding sites engineered into the DNA of interest. This method can be adapted to follow many recombinant DNAs in live cells over time.
Explore More Videos
Related Videos
15:00
Long-term Imaging Mammalian Cells using Wide-Field Microscopy
Related Videos
5201 Views
15:57
Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
Related Videos
22418 Views
08:21
Time-lapse Imaging of Mitosis After siRNA Transfection
Related Videos
16930 Views
10:27
A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
Related Videos
25410 Views
14:18
Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
Related Videos
21077 Views
06:52
Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast
Related Videos
20170 Views
10:34
FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
Related Videos
23021 Views
12:54
In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
Related Videos
13777 Views
08:14
Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
Related Videos
10991 Views
08:02
Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
Related Videos
9580 Views
Read Article
Cite this Article
Norby, K., Chiu, Y., Sugden, B. Monitoring Plasmid Replication in Live Mammalian Cells over Multiple Generations by Fluorescence Microscopy. J. Vis. Exp. (70), e4305, doi:10.3791/4305 (2012).
Copy