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Chapters
- 00:05Title
- 01:19Obtaining and Preparing the Tissue
- 02:22Mounting the Tissue
- 03:38Stimulation
- 04:31Harvesting
- 05:11Mucosal Explants Responses to Pro-Inflammatory Stimulus
- 05:58Conclusion
We introduce a novel method for the maintenance of human intestinal mucosa in culture and monitoring of the response to various types of stimuli over at least 24 hrs. With our method, the polarity of the tissue is maintained, allowing for a physiological stimulation via the apical route.
Tags
Human Intestinal Mucosa ExplantsIntestinal Micro-environmentImmunological ResponseTolerance Against Food AntigensImmune Responses Against Pathogenic MicrobesMicrobiota-host InteractionsMucus DegradationAntimicrobial PeptidesEpithelial BarrierGut-resident Lymphoid CellsIn Vitro AssaysMouse ModelsIrritable Bowel SyndromeInflammatory Bowel DiseaseColorectal Cancer
