Journal
/
Neuroscience
/
Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing

Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing

DOI:
10.3791/4451-v

•

10:32 min

•

January 11, 2013

•

, ,
1Behavioral Neuroscience,University at Albany

Chapters

  • 00:05Title
  • 01:28Cannulation and Set-up
  • 04:29Microdialysis Set-up and Equilibration
  • 06:33Sample Collection during Behavioral Testing
  • 08:55Hippocampal Glucose Levels during Behavioral Testing
  • 09:49Conclusion

Summary

Automatic Translation

Automatic Translation

Modulation of hippocampally-dependent spatial working memory by direct intrahippocampal microinjection, accompanied and followed by in vivo microdialysis for metabolites in conscious, behaving animals.

Tags

Hippocampal Insulin MicroinjectionIn Vivo MicrodialysisSpatial Memory TestingGlucose MetabolismNeural Activity Marker2-deoxyglucoseFunctional Magnetic Resonance ImagingAnesthesiaBehavioral MeasuresBrain State AlterationContinuous MeasurementBrain Extracellular Fluid ConcentrationsLactateMetabolitesAwake AnimalsUnrestrained AnimalsTasks Designed For Specific Brain RegionsAcute Pharmacological ManipulationHippocampal MeasurementsSpatial Working Memory TaskSpontaneous AlternationEnhanced GlycolysisIntrahippocampal Insulin AdministrationMemory ImprovementHippocampal GlycolysisMicrodialysis Probe DeliveryMetabolite MeasurementSpontaneous Alternation TaskHippocampal Function Measure

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/4451?article_type=v

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation
check_url/4451?article_type=v

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI
check_url/4451?article_type=v

Microdialysis of Ethanol During Operant Ethanol Self-administration and Ethanol Determination by Gas Chromatography
check_url/4451?article_type=v

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices
check_url/4451?article_type=v

In vivo Optogenetic Stimulation of the Rodent Central Nervous System
check_url/4451?article_type=v

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging
check_url/4451?article_type=v

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System
check_url/4451?article_type=v

Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders
check_url/4451?article_type=v

Assessing Spatial Learning and Memory in Small Squamate Reptiles
check_url/4451?article_type=v

An In Ovo Model for Testing Insulin-mimetic Compounds

Read Article