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Isometric and Eccentric Force Generation Assessment of Skeletal Muscles Isolated from Murine Models of Muscular Dystrophies
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Biology
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Isometric and Eccentric Force Generation Assessment of Skeletal Muscles Isolated from Murine Models of Muscular Dystrophies

Isometric and Eccentric Force Generation Assessment of Skeletal Muscles Isolated from Murine Models of Muscular Dystrophies

DOI:
10.3791/50036-v

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14:10 min

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January 31, 2013

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, , ,
1Department of Anatomy and Cell Biology,School of Dental Medicine, University of Pennsylvania, 2Department of Physiology,Perelman School of Medicine, University of Pennsylvania, 3Department of Anatomy and Cell Biology, School of Dental Medicine,School of Dental Medicine, University of Pennsylvania

Chapters

  • 00:05Title
  • 02:07EDL Dissection and Preparation
  • 04:21Diaphragm Dissection and Preparation
  • 05:57Mounting Muscles in Mechanics Bath
  • 06:51Isometric Contractions
  • 08:52Eccentric Contractions
  • 09:40Muscle Removal from the Apparatus
  • 10:59Results: Differences in Muscle Function Between Wildtype and Mdx Mice
  • 13:28Conclusion

Summary

Automatic Translation

Automatic Translation

Muscle function measurements contribute to the evaluation of potential therapeutics for muscle pathology, as well as to the determination of mechanisms underlying physiology of this tissue. We will demonstrate the preparation of the extensor digitorum longus and diaphragm muscles for functional testing. Protocols for isometric and eccentric contractions will be shown, as well as differences in results between dystrophic muscles, representing a pathological state, and wildtype muscles.

Tags

Keywords: Isometric ForceEccentric ForceSkeletal MuscleMuscular DystrophyExtensor Digitorum Longus (EDL)DiaphragmMuscle Function AssessmentSarcolemma StabilityMuscle PhysiologyPre-clinical TrialsMouse Models

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