Isometric and Eccentric Force Generation Assessment of Skeletal Muscles Isolated from Murine Models of Muscular Dystrophies

Catherine Moorwood; Min Liu; Zuozhen Tian; Elisabeth R. Barton

doi:10.3791/50036

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Isometric and Eccentric Force Generation Assessment of Skeletal Muscles Isolated from Murine Models of Muscular Dystrophies

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Isometric and Eccentric Force Generation Assessment of Skeletal Muscles Isolated from Murine Models of Muscular Dystrophies

32,725 Views

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14:10 min

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January 31, 2013

DOI:

10.3791/50036-v

DOI:

10.3791/50036-v

•

14:10 min

January 31, 2013

•

9 Views

Catherine Moorwood¹, Min Liu², Zuozhen Tian², Elisabeth R. Barton³

¹Department of Anatomy and Cell Biology,School of Dental Medicine, University of Pennsylvania, ²Department of Physiology,Perelman School of Medicine, University of Pennsylvania, ³Department of Anatomy and Cell Biology, School of Dental Medicine,School of Dental Medicine, University of Pennsylvania

Summary

Muscle function measurements contribute to the evaluation of potential therapeutics for muscle pathology, as well as to the determination of mechanisms underlying physiology of this tissue. We will demonstrate the preparation of the extensor digitorum longus and diaphragm muscles for functional testing. Protocols for isometric and eccentric contractions will be shown, as well as differences in results between dystrophic muscles, representing a pathological state, and wildtype muscles.