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Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos
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Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos

Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos

DOI:
10.3791/50057-v

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09:57 min

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January 30, 2013

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, , , , , , , ,
1Institut de Recherches Cliniques de Montréal (IRCM), 2Department of Biochemistry,Université de Montréal

Chapters

  • 00:05Title
  • 02:15Collection and Fixation of Drosophila Embryos
  • 04:44Post-fixation Processing
  • 05:58Hybridization and Post-hybridization Washes
  • 08:14FISH Showing Subcellular Localization of mRNA in Drosophila Embryos
  • 09:06Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a whole-mount fluorescent in situ hybridization (FISH) protocol for determining the expression and localization properties of RNAs expressed during embryogenesis in the fruit fly, Drosophila melanogaster.

Tags

Keywords: RNA In Situ HybridizationRNA-ISHFluorescent In Situ HybridizationFISHTyramide Signal AmplificationTSAGene ExpressionRNA LocalizationDrosophila EmbryosDevelopmental Biology

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