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JoVE Journal Biology
Genetic Modification and Recombination of Salivary Gland Organ Cultures
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Genetic Modification and Recombination of Salivary Gland Organ Cultures

Article DOI: 10.3791/50060
January 28th, 2013 •
*1, *1, 1, 1
1Department of Biological Sciences, University at Albany, SUNY
* These authors contributed equally

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January 28th, 2013

A technique to genetically manipulate epithelial cells within whole ex vivo cultured embryonic mouse submandibular glands (SMGs) using viral gene transfer is described. This method takes advantage of the innate ability of SMG epithelium and mesenchyme to spontaneously recombine after separation and infection of epithelial rudiments with adenoviral vectors.

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