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JoVE Journal
Biology

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Protein Transfection of Mouse Lung
 

Protein Transfection of Mouse Lung

Article DOI: 10.3791/50080
May 15th, 2013

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Summary May 15th, 2013

Transgenic mice or viral vectors have been used to increase protein expression within the lung. However, these techniques are time-consuming, technically challenging and have off-target effects that can confound results. Our protein transfection protocol uses a lipid based transfection reagent and an ultrafine microsprayer to uniformly deliver active protein to lung cells.

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