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December 26, 2012
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We present a protocol for freezing and cryosectioning yeast communities to observe internal patterns of fluorescent cells. The method relies on methanol-fixing and OCT-embedding to preserve the spatial distribution of cells without inactivating fluorescent proteins within a community.
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Momeni, B., Shou, W. Cryosectioning Yeast Communities for Examining Fluorescence Patterns. J. Vis. Exp. (70), e50101, doi:10.3791/50101 (2012).
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