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JoVE Journal
Biology

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Cryosectioning Yeast Communities for Examining Fluorescence Patterns
 

Cryosectioning Yeast Communities for Examining Fluorescence Patterns

Article DOI: 10.3791/50101
December 26th, 2012

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Summary December 26th, 2012

We present a protocol for freezing and cryosectioning yeast communities to observe internal patterns of fluorescent cells. The method relies on methanol-fixing and OCT-embedding to preserve the spatial distribution of cells without inactivating fluorescent proteins within a community.

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