Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo

Ayelet Kohl; Yoav Hadas; Avihu Klar; Dalit Sela-Donenfeld

doi:10.3791/50136

Journal

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Neuroscience

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Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo

10,783 Views

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10:04 min

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May 29, 2013

DOI:

10.3791/50136-v

DOI:

10.3791/50136-v

•

10:04 min

May 29, 2013

•

1 Views

Ayelet Kohl¹, Yoav Hadas², Avihu Klar², Dalit Sela-Donenfeld¹

¹Koret School of Veterinary Medicine,The Hebrew University of Jerusalem, ²Department of Medical Neurobiology,The Hebrew University of Jerusalem

Summary

How neuronal networks are established in the embryonic brain is a fundamental question in developmental neurobiology. Here we combined an electroporation technique with novel genetic tools, such as Cre/Lox–plasmids and PiggyBac-mediated DNA transposition system in the avian hindbrain to label dorsal interneurons and track their axonal projections and synaptic targets at various developmental stages.