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JoVE Journal Neuroscience
Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo
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Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo

Article DOI: 10.3791/50136 • 10:04 min • May 29th, 2013
May 29th, 2013 •
1, 2, 2, 1
1Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, 2Department of Medical Neurobiology, The Hebrew University of Jerusalem

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Summary
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How neuronal networks are established in the embryonic brain is a fundamental question in developmental neurobiology. Here we combined an electroporation technique with novel genetic tools, such as Cre/Lox–plasmids and PiggyBac-mediated DNA transposition system in the avian hindbrain to label dorsal interneurons and track their axonal projections and synaptic targets at various developmental stages.

Tags

Electroporation Hindbrain Axonal Trajectories Synaptic Targets Chick Embryo Neuronal Cells DNA Electroporation Avian Hindbrain Neuronal Progenitors Axonal Projections Developmental Stages Genetic Tools Enhancer Elements Cre/Lox - Based Plasmids PiggyBac-mediated DNA Transposition System GFP Expression Interneurons (dA1) Brainstem Regions Cell Targeting Circuit Formation
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