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Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

Article DOI: 10.3791/50141-v • 12:05 min • March 6th, 2013
March 6th, 2013 •
1,2, 1, 1,2, 1,2, 1,2, 1,2
1Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research, 2The University of Melbourne

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Summary
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Biophysical and biochemical studies of interactions among membrane-embedded protein domains face many technical challenges, the first of which is obtaining appropriate study material. This article describes a protocol for producing and purifying disulfide-stabilized transmembrane peptide complexes that are suitable for structural analysis by solution nuclear magnetic resonance (NMR) and other analytical applications.

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Disulfide-stabilized Transmembrane Peptide Complexes Structural Studies Lipid-embedded Alpha-helical Domains Membrane Proteins Folding And Assembly TM Signaling Regulation Cell-surface Protein Levels Biophysical Analysis Biochemical Analysis TM Peptide Complexes Hydrophobicity Peptide Chemistry Techniques Study Material Production Stable Helical Conformations Spontaneous Complex Formation Homo-dimeric TM Peptide Complexes Hetero-dimeric TM Peptide Complexes E. Coli Expression System Stable Isotope Labels Nuclear Magnetic Resonance (NMR) Non-natural Amino Acids
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