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JoVE Journal Chemistry
Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
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Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

Article DOI: 10.3791/50141 • 12:05 min
March 6th, 2013 •
1,2, 1, 1,2, 1,2, 1,2, 1,2
1Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research, 2The University of Melbourne

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March 6th, 2013

Biophysical and biochemical studies of interactions among membrane-embedded protein domains face many technical challenges, the first of which is obtaining appropriate study material. This article describes a protocol for producing and purifying disulfide-stabilized transmembrane peptide complexes that are suitable for structural analysis by solution nuclear magnetic resonance (NMR) and other analytical applications.

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