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Chemistry

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Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
 

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

Article doi: 10.3791/50141
March 6th, 2013

Summary March 6th, 2013

Biophysical and biochemical studies of interactions among membrane-embedded protein domains face many technical challenges, the first of which is obtaining appropriate study material. This article describes a protocol for producing and purifying disulfide-stabilized transmembrane peptide complexes that are suitable for structural analysis by solution nuclear magnetic resonance (NMR) and other analytical applications.

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