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High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli
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High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

Article DOI: 10.3791/51464 • 12:16 min • July 30th, 2014
July 30th, 2014 •
1, 2, 1, 1
1Architecture et Fonction des Macromolécules Biologiques (AFMB), Aix-Marseille Université, 2iBiTec-S, Service d'Ingénierie Moléculaire des Protéines (SIMOPRO), Commissariat à l'énergie atomique et aux énergies alternatives (CEA) Saclay, France

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A protocol for the quantitative, high throughput expression screening and analytical purification of fusion proteins from small-scale Escherichia coli cultures is described and applied to the expression of disulfide-rich animal venom protein targets.

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High Throughput Quantitative Expression Screening Purification Recombinant Disulfide-rich Venom Proteins E. Coli Protein Production Structural And Functional Studies Insoluble Expression Small-scale Cultures Soluble Expression High Throughput Protein Expression Screening Structural Genomics Programs Liquid Handling Robot Specialized Equipment Therapeutic Drug Leads Selective Proteins Complex Disulfide Bond Networks FP7 European Venomics Project
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