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外用转基因大鼠通过代<em>在子宫内</em>电穿孔和<em>在体内</em>生物发光筛选
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
DOI:

08:44 min

September 24, 2013

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Chapters

  • 00:05Title
  • 02:01In utero Electroporation (IUE)
  • 03:10Bioluminescence Live Imaging of the Enzymatic Luciferase Reaction
  • 05:47Analysis of Bioluminescence Images
  • 06:41Results: Luminescence Detection After Electroporation
  • 08:01Conclusion

Summary

Automatic Translation

基因可以在E16 在子宫内的电穿孔(IUE)的皮质或大鼠的海马通过的开发过程中进行操作,以使在神经元的连接的快速和有针对性的修改对于成年动物的行为或神经病理学的后来的研究。产后体内成像为IUE成功的控制是通过激活共转染的荧光素酶的生物发光进行的。

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