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Journal / Immunology and Infection / Ex Vivo Red Blood Cell Hemolyse Assay voor de…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Ex Vivo Red Blood Cell Hemolyse Assay voor de beoordeling van de pH-responsieve Endosomolytic Agenten voor Cytosolic Levering van Biomacromoleculaire Drugs
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Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
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Ex Vivo Red Blood Cell Hemolyse Assay voor de beoordeling van de pH-responsieve Endosomolytic Agenten voor Cytosolic Levering van Biomacromoleculaire Drugs

Article DOI: 10.3791/50166-v • 11:13 min • March 9th, 2013
March 9th, 2013 •
*1,2, *1,2, *1,2, 2,3, 1, 1,2, 4, 1,2,5,6, 1,2
1Department of Biomedical Engineering, Vanderbilt University, 2Vanderbilt Institute for Nanoscale Science & Engineering, Vanderbilt University, 3Interdisciplinary Materials Science Program, Vanderbilt University, 4Monroe Carell Jr. Children's Hospital, Vanderbilt University Medical Center, 5Department of Chemical & Biomolecular Engineering, Vanderbilt University, 6Department of Cancer Biology, Vanderbilt University
* These authors contributed equally

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Een hemolyse assay kan worden gebruikt als een snelle, high-throughput screen van drug delivery systems 'cytocompatibility en endosomolytic activiteit intracellulaire lading levering. De test meet de verstoring van de erythrocyten membranen als functie van pH milieu.

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Immunologie Cellulaire Biologie Geneeskunde Biomedische Technologie Bioengineering Kankerbiologie Moleculaire Biologie Erytrocyten endosomen kleine interfererende RNA Gene Therapy Nanomedicine Gene levering Nanodeeltjes endosoom Escape intracellulair verkeer Cytosolic Drug Delivery rood bloedcellen assay
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