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JoVE Journal
Immunology and Infection

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Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches
 

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches

Article DOI: 10.3791/50167
March 17th, 2013

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Summary March 17th, 2013

There is an increasing interest in understanding the immunological functions of specific subpopulations of cells in Peyer's patches (PPs), the primary inductive sites of gut-associated lymphoid tissues. Here we outline parallel protocols for preparing PP single cell preparations for flow cytometric analysis and PP cryosections for immunostaining.

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