Murine Spinotrapezius Model to Assess the Impact of Arteriolar Ligation on Microvascular Function and Remodeling

Journal

Medicine

JoVE Journal
Medicine
Author Produced

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JoVE Journal Medicine

Murine Spinotrapezius Model to Assess the Impact of Arteriolar Ligation on Microvascular Function and Remodeling

DOI:

10.3791/50218-v

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16:43 min

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March 03, 2013

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Alexander Michael Guendel*¹, Kyle S. Martin*¹, Joshua Cutts, Patricia L. Foley, Alexander M. Bailey, Feilim Mac Gabhann, Trevor R. Cardinal, Shayn M. Peirce

¹Department of Biomedical Engineering,University of Virginia, ²Department of Biomedical Engineering,California Polytechnic State University, ³Office of Animal Welfare,University of Virginia, ⁴Department of Biomedical Engineering & Institute for Computational Medicine,Johns Hopkins University

Chapters

00:01Title
01:34Introduction
03:48Surgical Instruments
04:02Mouse Preparation
04:47Ligation Procedure
09:25Intravital Microscopy and Functional Vasodilation
12:04Tissue Harvest
14:24Representative Results
16:15Conclusion

Summary

Automatic Translation

We demonstrate a novel arterial ligation model in murine spinotrapezius muscle, including a step-by-step procedure and description of required instrumentation. We describe the surgery and relevant outcome measurements relating to vascular network remodeling and functional vasodilation using intravital and confocal microscopy.

Murine Spinotrapezius Model to Assess the Impact of Arteriolar Ligation on Microvascular Function and Remodeling

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