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Live Cell Cycle Analysis of Drosophila Tissues using the Attune Acoustic Focusing Cytometer and Vybrant DyeCycle Violet DNA Stain

Article DOI: 10.3791/50239 • 11:00 min
May 19th, 2013 •
*1, *1, *1, 1, 1
1Molecular, Cellular and Developmental Biology, University of Michigan
* These authors contributed equally

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May 19th, 2013

A protocol for cell cycle analysis of live Drosophila tissues using the Attune Acoustic Focusing Cytometer is described. This protocol simultaneously provides information about relative cell size, cell number, DNA content and cell type via lineage tracing or tissue specific expression of fluorescent proteins in vivo.

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